Stereology determining sampling precision

#STEREOLOGY DETERMINING SAMPLING PRECISION HOW TO#
#STEREOLOGY DETERMINING SAMPLING PRECISION VERIFICATION#
#STEREOLOGY DETERMINING SAMPLING PRECISION SOFTWARE#

#STEREOLOGY DETERMINING SAMPLING PRECISION VERIFICATION#

Ann Anat 182:133вЂ“142ДЇarel PB (2002) Trust, but verify: the necessity of empirical verification in quantitative neurobiology. J Microsc 106:1вЂ“18Дolapchieva S, Eggers R, Kuhnel W (2000) Automatic image analysis of the postnatal growth of axons and myelin sheaths in the tibial and peroneal nerves of the rabbit.

J Microsc 106:19вЂ“32Д¬ruz Orive LM (1976b) Correction of stereological parameters from biased samples on nucleated particle phases. J Comp Neurol 364:6вЂ“15Д¬ruz Orive LM (1976a) Correction of stereological parameters from biased samples on nucleated particle phases. Trends Neurosci 15:9вЂ“13Д¬oggeshall RE, Lekan HA (1996) Methods for determining numbers of cells and synapses: a case for more uniform standards of review. Krieger, MalabarД¬oggeshall RE (1992) A consideration of neural counting methods. Trends Neurosci 24:11вЂ“17Д¬assel CM, Sarndal CE, Wretman JH (1993) Foundations of inference in sampling. Anat Rec 94:239вЂ“247Д«enes FM, Lange N (2001) Two-dimensional versus three-dimensional cell counting: a practical perspective.

This paper reviews the basic principles of the stereological tools from the first disector applications through some of the most recently devised methods.ДЄbercrombie M (1946) Estimation of nuclear population from microtome sections. To cope with this problem, over the last decades, a number of design-based stereological tools have been developed in order to obtain unbiased and reliable quantitative estimates of cell and tissue elements that originate from light microscopy images. Obviously, the number and size of profiles of an object do not correspond to the object number and size and thus significant mistakes can be made in the interpretation of the quantitative data obtained from profiles. Whereas counting and measuring histological elements may appear easy, especially today with the availability of dedicated software, in fact it is not, since what we can count and measure on light microscopy images are not the true histological elements but actually profiles of them. In addition, the measurement of several morphological parameters, such as the diameter of cells, the length of vessels, etc., can also be important for the quantitative assessment of the features of a tissue. The main objective is the count of objects, usually cells. Whether as an introduction to the process for students or as a refresher for experienced researchers, this practical guide is a perfect overview of a crucial but often overlooked step in empirical social work research.The quantitative investigation of images taken from light microscopy observation is one of the pillars of biological and biomedical investigation. He also offers strategies for mitigating pressures to increase sample size when doing so may not be feasible. In concise, example-rich chapters, Dattalo covers sample-size determination using power analysis, confidence intervals, computer-intensive strategies, and ethical or cost considerations, as well as techniques for advanced and emerging statistical strategies such as structural equation modeling, multilevel analysis, repeated measures MANOVA and repeated measures ANOVA.

#STEREOLOGY DETERMINING SAMPLING PRECISION HOW TO#

This pocket guide shows social work students, educators, and researchers how to prevent some of the mistakes that would result from a wrong sample size decision by describing and critiquing four main approaches to determining sample size.

#STEREOLOGY DETERMINING SAMPLING PRECISION SOFTWARE#

Computer programs help, but no single software package exists that allows researchers to determine sample size across all statistical procedures.

A researcher's decision about the sample to draw in a study may have an enormous impact on the results, and it rests on numerous statistical and practical considerations that can be difficult to juggle.

Stereology determining sampling precision